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Localization of cytokeratin 10 mRNA in human epidermis using nonradioactive in situ hybridization as a routine method

I Meinhold-Heerlein1, T Brandstetter, F Kommoss

  • 1Department of Gynecology and Obstetrics, School of Medicine, University of Freiburg, Germany. imeinh@frk1.ukl.uni-freiburg.de

Archives of Dermatological Research
|July 29, 1998
PubMed
Abstract

No abstract available in PubMed .

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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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