Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear structure--skeletal gene expression interrelationships

G S Stein1, A J van Wijnen, J L Stein

  • 1Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, 55 Lake Ave. North, Worcester, MA 01655, USA. Gary.Stein @banyan.ummed.edu

Frontiers in Bioscience : a Journal and Virtual Library
|July 31, 1998
PubMed
Summary

This study reveals how nuclear architecture influences bone-specific gene transcription, linking it to hormone control. It explores how chromatin and nuclear matrix organize regulatory signals for gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Genetic Determinants of Dental Maturation in Children.

Journal of dental research·2022
Same author

Microcomputed analysis of nerve angioarchitecture after combined stem cell delivery and surgical angiogenesis to nerve allograft.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2021
Same author

Higher order genomic organization and epigenetic control maintain cellular identity and prevent breast cancer.

Genes, chromosomes & cancer·2019
Same author

Micro-RNAS Regulate Metabolic Syndrome-induced Senescence in Porcine Adipose Tissue-derived Mesenchymal Stem Cells through the P16/MAPK Pathway.

Cell transplantation·2018
Same author

Treatment of Dupuytren's contracture: a systematic review.

The bone & joint journal·2018
Same author

Effectiveness of rosiglitazone in reducing flexion contracture in a rabbit model of arthrofibrosis with surgical capsular release: A biomechanical, histological, and genetic analysis.

Bone & joint research·2016

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Osteocalcin gene transcription is crucial for bone development and function.
  • Understanding gene regulation requires examining nuclear architecture and its role in controlling gene expression.
  • Steroid hormones significantly impact bone metabolism and gene activity.

Purpose of the Study:

  • To investigate the functional linkage between nuclear architecture and the regulation of bone tissue-specific transcription.
  • To evaluate the role of chromatin structure, nucleosome organization, and the nuclear matrix in integrating regulatory signals for the osteocalcin gene.
  • To elucidate mechanisms directing transcription factors to specific nuclear domains for gene expression.

Main Methods:

  • Utilizing the osteocalcin gene as a model system for bone-specific transcription.

Related Experiment Videos

  • Analyzing chromatin structure and nucleosome organization.
  • Investigating the nuclear matrix and its association with gene regulatory elements.
  • Evaluating the targeting of transcription factors to subnuclear domains.

    • Main Results:

    • Evidence for a functional link between nuclear architecture and developmental/steroid hormone-responsive control of osteocalcin gene transcription.
    • Demonstration of how chromatin structure and nuclear matrix contribute to integrating regulatory signals.
    • Identification of mechanisms that direct specific transcription factors to nuclear domains supporting gene expression.

    Conclusions:

    • Nuclear architecture plays a critical role in orchestrating tissue-specific gene transcription.
    • The interplay of chromatin, nucleosome organization, and the nuclear matrix is essential for precise gene regulation.
    • Specific subnuclear localization of transcription factors is a key mechanism for controlling gene expression in bone cells.