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Genetic variability among yellow fever virus 17D substrains
R Galler1, P R Post, C N Santos
1Departamento de Bioquimica e Biologia Molecular, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil. rgaller@gene.dbbm.fiocruz.br
Vaccine
|July 31, 1998
Summary
Researchers sequenced yellow fever virus (YFV) strains, identifying specific genetic changes linked to attenuation. The 17DD strain demonstrated the highest genetic stability among the studied YFV variants.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Yellow fever virus (YFV) poses a significant public health threat.
- Understanding genetic variations in YFV is crucial for vaccine development and viral attenuation studies.
Purpose of the Study:
- To determine the complete nucleotide genome sequences of two yellow fever virus strains, 17DD and 17D-213.
- To compare these sequences with other YFV strains, including the parental Asibi strain, to identify genetic differences related to attenuation and substrain specificity.
Main Methods:
- Whole genome sequencing of YFV strains 17DD and 17D-213.
- Comparative genomic analysis with existing YFV strain sequences (e.g., Asibi, 17D-204).
- Calculation of accumulated nucleotide changes per passage based on known passage histories.
Main Results:
- Identified 48 nucleotide sequence differences specific to the 17D strain, potentially linked to viral attenuation.
- Found 43 nucleotide differences that were substrain-specific among 17D substrains.
- Determined the number of nucleotide and amino acid substitutions between strains and calculated changes per passage.
- The 17DD strain exhibited the highest genetic stability based on accumulated changes per passage.
Conclusions:
- Specific nucleotide changes in the 17D strain may contribute to yellow fever virus attenuation.
- The 17DD strain is the most genetically stable among the analyzed yellow fever virus strains.
- Genomic sequencing and comparative analysis provide insights into viral evolution and attenuation mechanisms.