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Immunoglobulin E-mediated anaphylaxis activates nuclear factor kappaB in rat small intestine
J Carvalho Tavares1, Y Bayón, M Sánchez Crespo
1Instituto de Biología y Genética Molecular, CSIC, Facultad de Medicina, Valladolid, Spain.
Objective And Design:
Since IgE-dependent reactions induce the inducible isoform of NO synthase, we postulated the involvement of the transcription factor NF-kappaB.
Materials:
72 Wistar rats were divided into 6 groups and used to study the hemorrhagic necrosis of the small intestine elicited by anaphylaxis.
Treatment:
Passive anaphylaxis was produced by i.p. sensitization with IgE anti-dinitrophenyl monoclonal antibody and i.v. challenge with the cognate antigen.
Methods:
Competitive PCR was used to assay the expression of p50 subunit of NF-kappaB. kappaB-binding activity was assayed by electrophoretic mobility shift assay.
Results:
The PCR assay showed a time-dependent increase of mRNA coding for the p50 subunit of NF-kappaB, which was maximal 1 h after challenge (40+/-3, versus 230+/-32 fM, mean +/- SEM) and decreased to prechallenge level at 4h. kappaB-binding activity was also increased.
Conclusions:
IgE-mediated reactions trigger a pathway for nuclear signaling that seems to be related to the intermediate/late response of the allergic reaction.