Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

bca: an activation-related B-cell gene

L Gangi-Peterson1, S N Peterson, L H Shapiro

  • 1Curriculum in Genetics and Molecular Biology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.

Molecular Immunology
|July 31, 1998
PubMed
Summary

Researchers discovered a new B-cell gene (bca) involved in immune cell activation. This gene shows potential roles in B-cell signaling pathways and is upregulated in autoimmune conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of sea-level rise on physiological ecology of populations of a ground-dwelling ant.

PloS one·2020
Same author

Deep sequencing of the TCR-β repertoire of human forkhead box protein 3 (FoxP3)<sup>+</sup> and FoxP3<sup>-</sup> T cells suggests that they are completely distinct and non-overlapping.

Clinical and experimental immunology·2016
Same author

Urinary high-mobility group box-1 associates specifically with lupus nephritis class V.

Lupus·2016
Same author

Immune homeostasis, dysbiosis and therapeutic modulation of the gut microbiota.

Clinical and experimental immunology·2014
Same author

CD300b regulates the phagocytosis of apoptotic cells via phosphatidylserine recognition.

Cell death and differentiation·2014
Same author

Differential regulation of cell death programs in males and females by Poly (ADP-Ribose) Polymerase-1 and 17β estradiol.

Cell death & disease·2013

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • B-cell activation is crucial for adaptive immunity.
  • Signal transduction pathways regulate B-cell function.
  • Novel genes involved in B-cell signaling remain to be identified.

Purpose of the Study:

  • To identify and characterize novel genes involved in B-cell activation.
  • To investigate the expression patterns of a newly identified gene, bca.
  • To explore the potential role of bca in B-cell signal transduction.

Main Methods:

  • Differential hybridization screening of a murine B-cell cDNA library.
  • Sequence analysis to predict protein domains.
  • Northern blot analysis to determine gene expression patterns in various cell types and tissues.

Related Experiment Videos

Main Results:

  • A novel gene, bca, was identified with sequence similarity to SH2 and SH3 domains.
  • bca transcript (1.8 kb) was detected in B-cell lines but not in T-cell or non-lymphoid lines.
  • bca expression was low in resting cells but significantly increased in activated B cells and in autoimmune mouse strains.

Conclusions:

  • The bca gene encodes a novel protein potentially involved in B-cell signal transduction.
  • bca expression is linked to B-cell activation and may play a role in autoimmune diseases.
  • Further research is warranted to elucidate the precise function of bca in B-cell signaling.