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Updated: Jul 10, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

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Published on: November 8, 2006

Multicellular stalk-like structures in Saccharomyces cerevisiae

D Engelberg1, A Mimran, H Martinetto

  • 1Department of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. ENGELBER@vms.huji.ac.il

Journal of Bacteriology
|July 31, 1998
PubMed
Summary

UV-irradiated yeast cells form novel multicellular stalks. These structures, observed in various microorganisms, may aid in spore dispersal.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Developmental Biology

Background:

  • Multicellular organization is a complex process.
  • Yeast cells surviving UV irradiation exhibit unique colony growth patterns.
  • These patterns involve the formation of long, thin, vertically growing multicellular structures termed stalks.

Purpose of the Study:

  • To describe reproducible conditions for generating yeast stalks.
  • To investigate the genetic basis of stalk formation.
  • To explore the potential role and generalizability of stalk formation in microorganisms.

Main Methods:

  • Controlled UV irradiation of yeast cultures.
  • Culturing and microscopic observation of yeast colonies.
  • Testing of various yeast mutants affecting cell polarity, development, UV response, and signal transduction.
  • Comparative studies with other microbial species (Candida albicans, Schizosaccharomyces pombe, Escherichia coli).

Main Results:

  • Established reproducible conditions for large-scale yeast stalk formation.
  • Identified mutants in cell polarity, developmental pathways, UV response, and signal transduction that form stalks.
  • Demonstrated stalk formation in Candida albicans, Schizosaccharomyces pombe, and Escherichia coli.
  • Observed elevated sporulation frequency in diploid yeast stalks.

Conclusions:

  • Yeast stalk formation is a reproducible multicellular phenomenon.
  • Stalk formation is influenced by genetic factors related to cell polarity, development, and stress response.
  • Mechanical environmental forces may drive stalk formation.
  • Stalk formation appears to be a general microbial property.
  • Yeast stalks may play a role in spore dissemination.