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Sensitive detection of trace aluminium in biological tissues by confocal laser scanning microscopy after staining
A Uchiumi1, A Takatsu, Y Teraki
1National Institute of Material and Chemical Research, Ibaraki, Japan.
The Analyst
|July 31, 1998
Abstract:
This paper describes a method for the sensitive detection of aluminium in biological tissues by real time confocal laser scanning microscopy after staining with lumogallion. The method enabled detection of aluminium > or = 9 micrograms g-1 in bone and is more sensitive than the conventional histochemical methods with aluminium and solochrome azurine, etc. Lumogallion reacts specifically with aluminium to form a fluorescent complex so that the proposed method is useful for detection and identification of aluminium in tissues.