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Rheological modelling of leukocytes
R Tran-Son-Tay1, H C Kan, H S Udaykumar
1Department of Aerospace Engineering, Mechanics & Engineering Science, University of Florida, Gainesville 32611-6250, USA. rtst@euler.aero.ufl.edu
Medical & Biological Engineering & Computing
|July 31, 1998
Summary
This study models leukocyte dynamics using a three-layer Newtonian approach. The nucleus
Area of Science:
- Biophysics
- Computational Biology
- Cell Mechanics
Background:
- Leukocyte (white blood cell) rheology is complex, exhibiting both Newtonian and non-Newtonian behaviors.
- Existing models may not fully capture the dynamic recovery characteristics of leukocytes.
Purpose of the Study:
- To develop and validate a three-layer Newtonian model for leukocyte dynamic behavior.
- To investigate the critical factors influencing leukocyte deformation and recovery.
Main Methods:
- A combined Eulerian-Lagrangian computational method was employed.
- A three-layer model (membrane, cytoplasm, nucleus) was formulated.
- Pipette experiments with fluorescent microscopy were used for validation.
Main Results:
- The three-layer model accurately describes leukocyte recovery characteristics by considering viscosity and capillarity differences.
- The model reproduces both Newtonian and apparent non-Newtonian behaviors.
- The nucleus' high viscosity, deformation, recovery, and interfacial surface energy are critical for cell rheology.
Conclusions:
- The nucleus plays a pivotal role in leukocyte deformation and recovery dynamics.
- Accurate modeling of leukocyte rheology requires accounting for internal structure and interfacial properties.
- The developed computational model provides a robust framework for understanding leukocyte mechanics.