Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

QCM response to solvated, tethered macromolecules

N C Fawcett1, R D Craven, P Zhang

  • 1Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg 39406, USA.

Analytical Chemistry
|July 31, 1998
PubMed
Summary

Quartz crystal microbalance (QCM) frequency shifts with macromolecule binding in solution are complex. New experiments show that response depends on adlayer thickness and distance from the QCM surface, clarifying previous findings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-isotope analysis reveals that feasts in the Stonehenge environs and across Wessex drew people and animals from throughout Britain.

Science advances·2019
Same author

Obstetric ultrasound education for the developing world: A learning partnership with the World Federation for Ultrasound in Medicine and Biology.

Ultrasound (Leeds, England)·2016
Same author

Cytolytic activity against mycobacterial antigens: differences between haemophiliacs with and without HIV infection.

Haemophilia : the official journal of the World Federation of Hemophilia·2016
Same author

In synch but not in step: Circadian clock circuits regulating plasticity in daily rhythms.

Neuroscience·2016
Same author

Trauma systems: models of prehospital and inhospital care.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2016
Same author

Agreement between objective and subjective assessment of image quality in ultrasound abdominal aortic aneurism screening.

The British journal of radiology·2014

Area of Science:

  • Biophysics
  • Materials Science
  • Analytical Chemistry

Background:

  • Quartz crystal microbalance (QCM) frequency shifts during macromolecule binding in solution are inconsistently reported.
  • Discrepancies exist regarding the relationship between macromolecular mass and QCM resonance frequency shifts in wet versus dry states.

Purpose of the Study:

  • To re-examine previous M13 DNA QCM data and clarify the frequency-mass relationship.
  • To investigate the behavior of RNA duplexes on a QCM with an intervening polymer layer.
  • To elucidate the influence of adlayer thickness and distance from the QCM surface on mass detection.

Main Methods:

  • Analysis of existing M13 DNA QCM data.
  • Experimental oscillation of RNA duplexes in solution.

Related Experiment Videos

  • Utilizing a lossy polymer layer to isolate the QCM from solution properties.
  • Systematic variation of adlayer thickness and macromolecule properties.

    • Main Results:

    • M13 DNA data exhibit a reciprocal square root of mass relationship, not a linear one.
    • RNA duplexes on a QCM with a polymer layer show a linear response for constant adlayer thickness.
    • Response per unit mass decreases with increasing distance from the QCM surface.
    • Mass beyond the acoustic overlayer is decoupled from QCM response.

    Conclusions:

    • The relationship between QCM frequency shifts and bound mass is dependent on experimental conditions, including adlayer properties.
    • Previous linear models for wet QCM measurements are insufficient.
    • The findings provide a more accurate understanding of QCM operation in liquid environments for macromolecule detection.