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p53 protein expression in benign and malignant breast lesions
A Ioakim-Liossi1, C Markopoulos, P Karakitsos
1Department of Pathology, Medical School, University of Athens, Greece.
Acta Cytologica
|July 31, 1998
Summary
p53 protein expression in breast cancer imprints can help identify malignancy. This simple immunocytochemical method aids in detecting genetic alterations in ductal breast cancer.
Area of Science:
- Oncology
- Pathology
- Molecular Biology
Background:
- p53 protein is a tumor suppressor.
- Altered p53 expression is common in various cancers, including breast cancer.
- Immunocytochemistry is a valuable diagnostic tool in surgical pathology.
Purpose of the Study:
- To evaluate p53 protein expression in breast cancer imprints.
- To correlate p53 expression with histologic grade and clinical stage of ductal breast carcinoma.
- To assess the utility of p53 immunocytochemistry in routine cytologic analysis.
Main Methods:
- Immunocytochemical avidin-extravidin complex technique used.
- Monoclonal antibody p53 as primary antibody.
- Applied to imprints from 60 ductal breast carcinomas and 20 benign breast lesions.
Main Results:
- p53 protein expression observed in 45% of breast cancer cases.
- Significant correlation between p53 expression and histologic grade (1, 2, 3) and clinical stage (I, II, III).
- All benign breast lesions were negative for p53 protein.
Conclusions:
- Immunocytochemical p53 protein detection in cytologic samples is a straightforward method.
- This technique can be integrated into routine laboratories for breast cancer diagnosis.
- It aids in identifying genetic alterations in primary ductal breast cancer.