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The extracellular domain of CD4 receptor possesses a protein kinase activity
L A Yakubov1, Y Y Kit, V A Richter
1Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences. yakubov@niboch.nsc.ru
FEBS Letters
|July 31, 1998
Abstract:
The CD4 receptor of T-helper cells is an essential participant in immune response formation and HIV infection. We report here that the extracellular domains of CD4 receptor can catalyze the phosphotransferase (kinase) reaction. Incubation of rsCD4 in solution with [gamma-32P]ATP results in the Ca2+-dependent autophosphorylation of the protein presumably at a His residue because the reaction is prevented by the diethylpyrocarbonate treatment. The rsCD4 phosphorylates milk casein or human plasma proteins as a Ser/Thr protein kinase.