[Monoclonal antibodies which recognize herpes simplex virus and their possible diagnostic application]

Area of Science:

• Immunology
• Virology
• Biotechnology

Background:

• Herpes simplex virus (HSV) infections are a significant public health concern.
• Accurate and rapid diagnostic tools are crucial for managing HSV infections.
• Monoclonal antibodies offer high specificity for viral detection.

Purpose of the Study:

• To develop and characterize monoclonal antibodies for the detection of HSV-1 and HSV-2.
• To evaluate the diagnostic potential of these antibodies in clinical specimens.

Main Methods:

• Conventional cellular fusion technology was employed to generate monoclonal antibodies.
• Indirect immunofluorescence assays were used for antibody screening and characterization.
• Neutralizing capacity of selected antibodies was assessed.
• Clinical specimens were analyzed using indirect immunofluorescence with the developed antibodies.

Main Results:

• Monoclonal antibodies capable of recognizing both HSV-1 and HSV-2 were successfully obtained.
• Antibodies 278-F7 and 70-G10 demonstrated significant neutralizing capacity.
• These antibodies effectively identified HSV-1 in clinical specimens up to a dilution of 1:2560 via indirect immunofluorescence.

Conclusions:

• The developed monoclonal antibodies are specific and sensitive tools for HSV detection.
• These antibodies can be reliably used for identifying HSV-1 and HSV-2 in clinical samples.
• The findings support the use of these monoclonal antibodies in diagnostic virology for HSV infections.