Vacancy affinity capillary electrophoresis to study competitive protein-drug binding
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Updated: Sep 10, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Published on: April 17, 2017
1Amsterdam Institute for Molecular Studies, Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
Abstract:
The suitability of the vacancy affinity capillary electrophoretic method (VACE) for study of displacement of a target drug from a protein by simultaneously administered drugs was investigated. As test system, the displacement of warfarin from bovine serum albumin (BSA) by furosemide and phenylbutazone was selected. It appears that the displacement can be observed well from the shift of the actual mobility of warfarin when a displacer drug is added. Also, the competitive action of the displacer drugs (affinity for BSA) is clearly visible. The VACE method seems to be attractive for rapid assessment of information about the competitive properties of coadministered compounds.
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