Ribosome-inactivating proteins (RIPs) of wild Oregon cucumber (Marah oreganus)
N J Shih1, K A McDonald, T Girbés
1Department of Chemical Engineering and Materials Science, University of California Davis, 95616, USA.
Abstract:
Two type 1 RIPs, designated as MOR-I and MOR-II, have been isolated from Marah oreganus (manroot) seed extract. They are similar but not identical to trichosanthin, a type 1 RIP in the same family. MOR-I and MOR-II are monomeric proteins with molecular weights of 27989.0 and 27632.8 respectively and have pI values greater than 8.8. MOR-I and MOR-II inhibit cell-free protein synthesis with IC50s of 0.063 and 0.071 nM, respectively, and are relatively stable with respect to temperature and pH variations. They share a conserved N-terminal amino acid sequence (D-SF-LS) and cross-react with goat anti-trichosanthin polyclonal serum.
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