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Fungal biotransformation of 6-nitrochrysene
J V Pothuluri1, J B Sutherland, J P Freeman
1National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079, USA. Jpothuluri@nctr.fda.gov
Applied and Environmental Microbiology
|August 4, 1998
Abstract:
The fungus Cunninghamella elegans was used to biotransform 6-nitrochrysene, a mutagen that is a widespread environmental contaminant. After 6 days, 74% of the 3H-labeled 6-nitrochrysene added had been metabolized to two isomeric sulfate conjugates. These conjugates were separated by high-performance liquid chromatography and identified by UV-visible, 1H nuclear magnetic resonance, and mass spectral techniques as 6-nitrochrysene 1-sulfate and 6-nitrochrysene 2-sulfate.