Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human immunodeficiency virus type 1 reverse transcriptase expressing the K70E mutation exhibits a decrease in

M D Miller1, P D Lamy, M D Fuller

  • 1Gilead Sciences, Foster City, California 94404, USA. michael_miller@gilead.com

Molecular Pharmacology
|August 4, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relationships between physically effective undegraded neutral detergent fiber and rumen fermentable starch in diets for lactating Holstein dairy cows.

Journal of dairy science·2026
Same author

Relationships between undegraded and physically effective fiber in diets for lactating Holstein dairy cows.

Journal of dairy science·2026
Same author

Influence of fiber degradability of corn silage in diets with lower and higher fiber content on lactational performance, nutrient digestibility, and ruminal characteristics in lactating Holstein cows.

Journal of dairy science·2020
Same author

Evaluation of source of corn silage and trace minerals on rumen characteristics and passage rate of Holstein cows.

Journal of dairy science·2020
Same author

Evaluation of source of corn silage and trace minerals on lactational performance and total-tract nutrient digestibility in Holstein cows.

Journal of dairy science·2020
Same author

A systematic review of community-based interventions for the treatment of adolescents with overweight and obesity.

Obesity reviews : an official journal of the International Association for the Study of Obesity·2018

Adefovir dipivoxil resistance in HIV-1 can be caused by reverse transcriptase mutations. The K70E mutation confers minor resistance to adefovir and 3TC, impacting viral replication.

Area of Science:

  • Virology
  • Molecular Biology
  • Drug Resistance

Background:

  • Adefovir dipivoxil (bis-POM PMEA) is an oral prodrug of adefovir (PMEA) for HIV-1 treatment.
  • HIV-1 reverse transcriptase (RT) mutations K65R and K70E reduce sensitivity to PMEA.
  • The K70E mutation is associated with impaired in vitro viral replication capacity.

Purpose of the Study:

  • To investigate the molecular mechanisms of adefovir (PMEA) resistance in HIV-1.
  • To characterize the enzymatic properties of HIV-1 RT mutants K65R, K70E, and M184V.

Main Methods:

  • Cloning, expression, and purification of recombinant HIV-1 RT enzymes with K65R, K70E, and M184V mutations.
  • Determination of kinetic parameters (Km, Ki) for dNTPs and inhibitors.
  • Evaluation of specific activity and processivity of mutant RT enzymes.

Related Experiment Videos

Main Results:

  • The K70E mutant showed a 2-3 fold increased Ki for PMEA diphosphate and 3TC triphosphate.
  • All tested mutants (K65R, K70E, M184V) exhibited reduced specific activity compared to wild-type RT.
  • The K70E mutant showed slightly impaired processivity, consistent with reduced in vitro replication.

Conclusions:

  • The K70E mutation confers minor resistance to adefovir (PMEA) and 3TC in HIV-1.
  • The K70E mutation is associated with reduced viral replication capacity.
  • Understanding these resistance mechanisms is crucial for optimizing HIV-1 treatment strategies.