Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid HLA-D matching method using PHA blasts as responding cells (preliminary data on PHA blasts HLA-D typing)

C Mawas, D Charmot, A Comoy

    Tissue Antigens
    |August 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The phospholipase A(2) inhibitor methyl indoxam suppresses diet-induced obesity and glucose intolerance in mice.

    British journal of pharmacology·2009
    Same author

    [Granulysin: antimicrobial molecule of innate and acquired immunity in human tuberculosis].

    Pathologie-biologie·2005
    Same author

    Interferon-gamma-induced membrane PAF-receptor expression confers tumor cell susceptibility to NK perforin-dependent lysis.

    Blood·2000
    Same author

    [Granzyme B: an essential protease for the inflammatory response].

    Pathologie-biologie·1999
    Same author

    Perforin and granzyme B lytic protein expression during chronic viral and autoimmune hepatitis.

    Liver·1998
    Same author

    Down-regulation of human granzyme B expression by glucocorticoids. Dexamethasone inhibits binding to the Ikaros and AP-1 regulatory elements of the granzyme B promoter.

    The Journal of biological chemistry·1998

    PHA-stimulated lymphocytes enable rapid HLA-D matching within 24-48 hours. This method requires fewer cells and offers a faster alternative for mixed lymphocyte reaction testing.

    Area of Science:

    • Immunology
    • Transplantation Immunology
    • Histocompatibility Testing

    Background:

    • Mixed lymphocyte reaction (MLR) is crucial for HLA-D matching in transplantation.
    • Traditional MLR requires significant time and cell numbers for accurate results.

    Purpose of the Study:

    • To evaluate a rapid method for HLA-D matching using PHA-stimulated lymphocytes.
    • To assess the feasibility of early MLR reading for potential donor matching.

    Main Methods:

    • Utilized phytohemagglutinin (PHA)-stimulated lymphocytes for mixed lymphocyte reaction (MLR).
    • Assessed response against HLA-D without latency, enabling readings within 24-48 hours.
    • Employed a one-way reaction with a reduced number of responding cells (5 x 10^3).

    Related Experiment Videos

    Main Results:

    • PHA-stimulated lymphocytes responded to HLA-D without latency, allowing MLR readings in 24-48 hours.
    • The method requires a minute number of responding cells (5 x 10^3).
    • HLA-D matching with frozen, mitogen-stimulated recipient cells was feasible within 24-48 hours.

    Conclusions:

    • This rapid MLR method offers a significant improvement over traditional HLA-D typing.
    • Preliminary data show encouraging concordance with classical HLA-D typing.
    • Further large-scale testing is warranted to validate this accelerated approach for donor matching.