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Related Experiment Videos

Simple screening test for significant bacteriuria in urine. Catalase determination by disk flotation method

Y Taguchi, B Grad

    Urology
    |September 1, 1976
    PubMed
    Summary

    The disk flotation test for catalase determination is a successful screening tool for significant bacteriuria, accurately identifying positive and negative urine samples with minimal false results.

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    Area of Science:

    • Clinical microbiology
    • Diagnostic testing

    Background:

    • Accurate detection of significant bacteriuria is crucial for patient management.
    • Traditional urine culture methods can be time-consuming.
    • Developing rapid screening tests for bacteriuria is an ongoing area of research.

    Purpose of the Study:

    • To evaluate the efficacy of catalase determination using the disk flotation method as a screening test for significant bacteriuria.
    • To compare the results of the disk flotation test with standard colony count cultures.

    Main Methods:

    • 294 urine samples were analyzed using both colony count cultures and catalase determinations via the disk flotation method.
    • Samples were categorized as positive or negative based on colony count thresholds.
    • False positive and false negative rates for the catalase test were assessed.

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    Main Results:

    • The disk flotation catalase test demonstrated success as a screening test for significant bacteriuria.
    • 36 specimens were positive by both methods.
    • 135 specimens were negative by both methods.
    • False negatives were associated with low colony counts (<6,000 colonies/ml).
    • Over 50% of false positives were linked to the presence of red blood cells.

    Conclusions:

    • Catalase determination by the disk flotation method is a viable and successful screening test for significant bacteriuria.
    • The test shows high concordance with traditional culture methods.
    • Further investigation is needed to elucidate the causes of remaining false positive results.