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Related Experiment Videos

Aqueous vascular endothelial growth factor and basic fibroblast growth factor decrease during regression of rabbit

T Yanagawa1, T Matsuo, N Matsuo

  • 1Department of Ophthalmology, Okayama University Medical School, Okayama City, Japan.

Japanese Journal of Ophthalmology
|August 5, 1998
PubMed
Summary
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Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) levels in rabbit aqueous humor decreased significantly during pupillary membrane regression. These growth factors may play a role in eye development and pupillary membrane maintenance.

Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Molecular Biology

Background:

  • The pupillary membrane is a transient vascular structure present in early ocular development.
  • Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are key regulators of angiogenesis and tissue development.

Purpose of the Study:

  • To investigate the roles of VEGF and bFGF in the aqueous humor during the regression of the pupillary membrane in rabbits.
  • To correlate growth factor levels with the morphological changes of the pupillary membrane.

Main Methods:

  • Aqueous humor samples were collected from newborn rabbits at 12, 20, and 60 days of age.
  • Quantitative sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure VEGF and bFGF concentrations.
  • Light microscopy was employed to examine the pupillary membrane structure at different ages.

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Main Results:

  • Aqueous VEGF levels significantly decreased from 12 days (median 752.5 pg/mL) to 60 days (median 190.5 pg/mL) of age (P < 0.0001).
  • Aqueous bFGF levels significantly decreased from 12 days (median 167.4 pg/mL) to 60 days (median 63.1 pg/mL) of age (P < 0.0001).
  • Microscopic analysis showed a rich capillary network in the pupillary membrane at 12 days, which regressed and disappeared by 60 days.

Conclusions:

  • Decreased levels of VEGF and bFGF correlate with the regression and disappearance of the pupillary membrane.
  • VEGF and bFGF likely play crucial roles in the maintenance and development of the pupillary membrane during ocular development.