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Ocular tissue concentrations of mitomycin C with variable dose and duration of application time in rabbits
1Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Abstract:
We measured mitomycin C (MMC) concentrations in ocular tissues in rabbits with variable dose (0.1, 0.2, or 0.4 mg) and duration of application time (1, 3, or 5 minutes) of MMC using high-performance liquid chromatography. Mitomycin C concentrations at the administered site after single subconjunctival application of MMC and after irrigation showed significant correlation with dose and duration of time of application. By multiple regression analysis, MMC concentrations (microg/g) at the conjunctiva were described as -6.73 + 67.4 x Dose (mg) + 1.66 x Time (minutes) (R2 0.65); at the sclera, -1.85 + 38.2 x Dose + 0.927 x Time (R2 0.63); at the cornea, -0.727 + 8.44 x Dose (R2 0.46). With a 0.2-mg MMC dose, in all three application times (1, 3, or 5 minutes), MMC concentrations in the conjunctiva at the administered quadrant were three times higher than in the neighboring quadrants and 6 to 7 times higher than in the opposite quadrant. In the sclera, MMC concentrations were 3.5 times higher than in the neighboring sites and over 8 to 9 times higher than in the opposite site. In the cornea, MMC concentrations were 2 to 3 times higher than in the neighboring sites and opposite site. In the iris-ciliary body, MMC concentrations were 0.61 microg/g at the administered site with 0.2 mg for 3-minute application, 2 times higher than in neighboring sites, and 2 times higher than in opposite sites.
Insights
Mitomycin C (MMC) ocular tissue concentrations in rabbits depend on dose and application time. Higher MMC doses and longer application times result in increased concentrations in ocular tissues like the conjunctiva and sclera.
Area of Science:
- Ophthalmology
- Pharmacology
- Biochemistry
Background:
- Mitomycin C (MMC) is an alkylating agent used in ophthalmic procedures.
- Understanding MMC distribution in ocular tissues is crucial for optimizing treatment and minimizing side effects.
Purpose of the Study:
- To quantify Mitomycin C (MMC) concentrations in various rabbit ocular tissues.
- To determine the influence of MMC dose and application duration on tissue concentrations.
Main Methods:
- High-performance liquid chromatography (HPLC) was used to measure MMC concentrations.
- Experiments involved variable MMC doses (0.1, 0.2, 0.4 mg) and application times (1, 3, 5 minutes) in rabbits.
Main Results:
- MMC concentrations correlated significantly with dose and application time.
- Regression models described MMC levels in conjunctiva, sclera, and cornea.
- Higher concentrations were observed at the application site compared to adjacent and opposite sites.
Conclusions:
- Ocular tissue MMC concentrations are dose- and time-dependent.
- Localized application leads to higher concentrations at the site of administration.
- Findings aid in refining MMC application protocols in ophthalmology.