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Purification of extracellular catalase from Aspergillus niger
J Rogalski1, J Fiedurek, A Gromada
1Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Acta Microbiologica Polonica
|August 6, 1998
Abstract:
The extracellular catalase (EC 1.11.1.6) produced by Aspergillus niger culture in 5-liter fermentor was isolated and purified by ion-exchange chromatography on DEAE Sepharose (fast flow) column, hydrophobic interaction on phenyl-Sepharose column and chromatofocusing on PBE 94 column. Some physico-chemical properties of two purified catalase forms were also determined (molecular weight, isolelectric point, polysaccharides contents, Km, and Ea).