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Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic, ristocetin A, chiral
K H Ekborg-Ott1, Y Liu, D W Armstrong
1Department of Chemistry, University of Missouri-Rolla 65401-0249, USA.
Abstract:
The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed-phase mode, the normal-phase mode, or the polar-organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase to another.