Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sphingomyelinase activity in human platelets

C G Simon1, S Chatterjee, A R Gear

  • 1Biochemistry Department, University of Virginia, School of Medicine, Charlottesville 22908, USA.

Thrombosis Research
|August 6, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Orthogonal and complementary measurements of properties of drug products containing nanomaterials.

Journal of controlled release : official journal of the Controlled Release Society·2022
Same author

A method for the evaluation of thousands of automated 3D stem cell segmentations.

Journal of microscopy·2015
Same author

Imaging cells on polymer spherulites.

Journal of microscopy·2004
Same author

Local anesthetic actions on thromboxane-induced platelet aggregation.

Anesthesia and analgesia·2001
Same author

ADP receptor antagonists inhibit platelet aggregation induced by the chemokines SDF-1, MDC and TARC.

FEBS letters·2001
Same author

Adenosine diphosphate strongly potentiates the ability of the chemokines MDC, TARC, and SDF-1 to stimulate platelet function.

Blood·2001
Same journal

Apoptotic versus procoagulant platelets: similar "necrotic" phenotype and procoagulant activity in vitro, but distinct adhesive protein composition.

Thrombosis research·2026
Same journal

Heatstroke-induced coagulopathy: A scoping review of therapeutic strategies and outcome reporting.

Thrombosis research·2026
Same journal

Mapping thrombus habitat: Non-contrast MRI radiomics and pixel-tile histomics approach to track venous thrombosis evolution in mice.

Thrombosis research·2026
Same journal

A study protocol for a randomised controlled trial evaluating the safety and efficiency of the YEARS algorithm versus computed tomography pulmonary angiography only for suspected acute pulmonary embolism in patients with cancer: the Hydra Study.

Thrombosis research·2026
Same journal

Associating the phenotypic expression of platelets with disease type through image-based single-cell profiling.

Thrombosis research·2026
Same journal

The mechanisms of contractile dysfunction following chronic limited platelet activation in (pro)thrombotic conditions.

Thrombosis research·2026
See all related articles

Human platelets contain acid-sphingomyelinase, an enzyme crucial for sphingolipid metabolism. Thrombin activation causes this enzyme to be secreted, influencing platelet function and aggregation.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Hematology

Background:

  • Sphingolipids like ceramide, sphingosine, and sphingosine-1-phosphate regulate critical cell functions.
  • Sphingosine-1-phosphate released from activated platelets potentiates aggregation.
  • Thrombin activation increases platelet sphingosine levels.

Purpose of the Study:

  • To determine if human platelets possess sphingomyelinase.
  • To investigate the regulation of platelet sphingomyelinase during platelet activation.

Main Methods:

  • Assayed sphingomyelinase activity using radioactive sphingomyelin as a substrate across a pH range of 4-10.
  • Investigated the effect of thrombin and ADP on platelet sphingomyelinase activity.
  • Measured enzyme secretion into the media following thrombin activation.

Related Experiment Videos

Main Results:

  • Human platelets contain an acid-sphingomyelinase with optimal activity at pH 5.0-5.5.
  • Enzyme activity was not significantly affected by Mg++, Ca++, Zn++, or EDTA.
  • Platelet activation by thrombin or ADP did not alter intracellular sphingomyelinase activity.
  • Thrombin stimulation led to the secretion of acid-sphingomyelinase activity into the extracellular medium.

Conclusions:

  • Human platelets possess a functional acid-sphingomyelinase.
  • Thrombin-induced platelet activation results in the secretion of this enzyme.
  • Secreted acid-sphingomyelinase may play a role in platelet signaling and aggregation modulation.