Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Female mouse germ cells form synchronously dividing cysts

M E Pepling1, A C Spradling

  • 1Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA.

Development (Cambridge, England)
|August 7, 1998
PubMed
Summary

Female mouse germ cells form interconnected clusters, similar to invertebrate germline cysts. These clusters exhibit synchronous cell division, suggesting a conserved mechanism for oocyte development in mammals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stem cells and their niches: integrated units that maintain Drosophila tissues.

Cold Spring Harbor symposia on quantitative biology·2008
Same author

The receptor-like tyrosine phosphatase lar is required for epithelial planar polarity and for axis determination within drosophila ovarian follicles.

Development (Cambridge, England)·2001
Same author

The nuclear location and chromatin organization of active chorion amplification origins.

Chromosoma·2001
Same author

Mouse ovarian germ cell cysts undergo programmed breakdown to form primordial follicles.

Developmental biology·2001
Same author

Stem cells and their progeny respond to nutritional changes during Drosophila oogenesis.

Developmental biology·2001
Same author

A niche maintaining germ line stem cells in the Drosophila ovary.

Science (New York, N.Y.)·2000

Area of Science:

  • Developmental biology
  • Cell biology
  • Reproductive biology

Background:

  • Oocyte development in invertebrates often involves interconnected cell cysts formed by synchronous cell divisions.
  • The organization of early female mouse germ cells into clusters suggests potential similarities to invertebrate germline cysts.
  • Understanding germ cell organization is crucial for reproductive health and fertility studies.

Purpose of the Study:

  • To investigate whether early female mouse germ cell clusters share characteristics with invertebrate germline cysts.
  • To determine if germ cell clusters in mouse embryos are formed through conserved developmental processes.

Main Methods:

  • Ovaries from mouse embryos (11.5-17.5 days postcoitum) were examined.
  • Confocal microscopy was used to visualize germ cell clusters and intercellular bridges.

Related Experiment Videos

  • Microtubules and organelles within intercellular bridges were analyzed.

    • Main Results:

    • Mouse germ cells in embryonic ovaries are connected by intercellular bridges containing microtubules and organelles.
    • Cells within these mouse germ cell clusters divide synchronously.
    • The number of cells in mouse clusters often corresponds to powers of two, mirroring invertebrate cyst formation.

    Conclusions:

    • Female mouse germ cell clusters display key features of invertebrate germline cysts.
    • Germline cyst formation appears to be a conserved process between invertebrates and mammals.
    • This conserved mechanism highlights fundamental aspects of oocyte development across species.