Related Experiment Video
Updated: Jan 13, 2026
A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1
Published on: July 22, 2022
Subconjunctival injection of low doses of mitomycin C: effects on fibroblast proliferation
I Pinilla1, J M Larrosa, V Polo
1Department of Ophthalmology, Miguel Servet Hospital, Zaragoza, Spain. sope.pini@sendanet.es
Abstract:
We evaluated the effect of low doses of mitomycin C (MMC) in subconjunctival injection on fibroblast viability. Eighteen rabbits received subconjunctival injections: group 1 was injected with balanced salt solution (BSS), group 2 received MMC 0.025 mg, and group 3 MMC 0.005 mg. Tissue samples were taken 1 h after the injection (conjunctiva, sclera and cornea at 0 and 90 degrees) and cultured. The average scleral and conjunctival outgrowth in the MMC-treated groups was significantly inferior to the outgrowth of the samples treated with BSS. Subconjunctival injections of MMC (0. 025 and 0.005 mg) have an inhibitory effect on conjunctiva at 0 degrees and on scleral fibroblast populations.
Insights
Low doses of mitomycin C (MMC) via subconjunctival injection significantly inhibit fibroblast populations in rabbit conjunctiva and sclera. This finding is crucial for understanding MMC
Area of Science:
- Ophthalmology
- Cell Biology
- Pharmacology
Background:
- Mitomycin C (MMC) is used in ophthalmic surgery.
- Its effect on fibroblast viability after subconjunctival injection requires detailed evaluation.
Purpose of the Study:
- To assess the impact of low-dose subconjunctival mitomycin C (MMC) on fibroblast viability in rabbit ocular tissues.
Main Methods:
- Subconjunctival injections of balanced salt solution (BSS), 0.025 mg MMC, or 0.005 mg MMC were administered to rabbits.
- Conjunctival, scleral, and corneal tissue samples were collected 1 hour post-injection.
- Fibroblast outgrowth was measured in cultured tissue samples.
Main Results:
- MMC-treated groups showed significantly reduced scleral and conjunctival fibroblast outgrowth compared to the BSS control group.
- Subconjunctival MMC demonstrated an inhibitory effect on conjunctival fibroblasts at 0 degrees and scleral fibroblast populations.
Conclusions:
- Low-dose subconjunctival mitomycin C (MMC) exhibits significant fibroblast inhibitory effects on conjunctiva and sclera.
- These findings are important for understanding the local tissue effects of MMC in ophthalmology.
