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Expression and distribution of kallikrein-binding protein mRNA in rat ocular tissues
M Murata1, M Nakagawa, S Takahashi
1Department of Ophthalmology, Yamagata University School of Medicine, Yamagata, Japan.
Abstract:
Kallikrein-binding protein (KBP) has recently been characterized as an endogenous inhibitor of kallikrein, and it is considered to be involved in several pathophysiological processes, such as hypertension. In the eye, its source has not been determined. We have investigated KBP mRNA expression in the ocular tissues of 5 Wistar-Kyoto rats using the technique of reverse transcription with polymerase chain reaction. Five cornea, sclera, iris, ciliary body and choroid samples were negative for KBP. Four retina samples were positive. KBP mRNA was more significantly expressed in the retina than in the other ocular tissues (p = 0.0238<0.05). Our present study demonstrates that KBP is synthesized in the rat retina, suggesting that KBP alteration may be involved in the retinal circulation.
Insights
Kallikrein-binding protein (KBP) is synthesized in the rat retina. This finding suggests KBP may play a role in retinal circulation and related conditions.
Area of Science:
- Ocular biology
- Molecular biology
- Biochemistry
Background:
- Kallikrein-binding protein (KBP) is an endogenous kallikrein inhibitor.
- KBP is implicated in pathophysiological processes like hypertension.
- The ocular source of KBP has not been previously identified.
Purpose of the Study:
- To investigate the expression and localization of KBP mRNA in rat ocular tissues.
- To determine if the retina synthesizes KBP.
Main Methods:
- Reverse transcription with polymerase chain reaction (RT-PCR) was used.
- Ocular tissues from Wistar-Kyoto rats were analyzed, including cornea, sclera, iris, ciliary body, choroid, and retina.
Main Results:
- KBP mRNA was not detected in the cornea, sclera, iris, ciliary body, or choroid.
- KBP mRNA was significantly expressed in the retina (p = 0.0238).
Conclusions:
- KBP is synthesized in the rat retina.
- KBP expression in the retina suggests its potential involvement in retinal circulation and associated pathologies.