Related Experiment Videos
Programmed cell death in the mouse cochleovestibular ganglion during development
K Nishizaki1, M Anniko, Y Orita
1Department of Otorhinolaryngology, Okayama University Medical School, Okayama, Japan. nishizak@cc.okayama-u.ac.jp
Summary
Programmed cell death (PCD) is crucial for development. This study tracked PCD in mouse cochleovestibular ganglion development, finding it regulates cell numbers during innervation and mitosis.
Area of Science:
- Developmental Biology
- Neuroscience
- Cell Biology
Background:
- Programmed cell death (PCD) is vital for organismal development, alongside cell proliferation.
- Understanding the precise timing and location of PCD is key to deciphering developmental processes.
Purpose of the Study:
- To investigate the spatiotemporal patterns of PCD, specifically apoptosis, during the development of the mouse cochleovestibular ganglion.
Main Methods:
- A time-sequence study was conducted on developing mouse cochleovestibular ganglia.
- The TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) method was employed for histochemical detection of apoptosis.
Main Results:
- Apoptosis was observed in the cochleovestibular ganglion between embryonic day 11.5 and 18.5.
- Peak apoptotic activity occurred in vestibular ganglion cells around embryonic day 13.5 and in spiral ganglion cells between days 15.5 and 16.5.
- These peaks coincided with critical developmental events like innervation and terminal mitosis.
Conclusions:
- Programmed cell death plays a significant role in the innervation process of the cochleovestibular ganglion.
- PCD appears to modulate the cell population size, particularly in response to overproduction from terminal mitosis.