Dissolved oxygen determination by electrocatalysed chemiluminescence with in-line solid phase media

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biochemistry

Background:

• Accurate dissolved oxygen (DO) measurement is crucial for aquatic ecosystem health.
• Existing methods may have limitations in sensitivity or real-time application.
• Developing novel sensing techniques is essential for effective environmental monitoring.

Purpose of the Study:

• To develop and validate a new method for quantifying dissolved oxygen in aqueous streams.
• To utilize enzymatic catalysis and chemiluminescence for sensitive oxygen detection.
• To establish a reliable method for dissolved oxygen analysis in the 0-10 mg/L range.

Main Methods:

• Employed glucose oxidase to catalyze the reaction between D-glucose and dissolved oxygen, producing hydrogen peroxide.
• Quantified hydrogen peroxide via luminol chemiluminescence.
• Incorporated in-line solid-phase media for pH adjustment and controlled luminophore release.
• Initiated the reaction using electrochemical catalysis.

Main Results:

• Chemiluminescence intensity was directly proportional to dissolved oxygen concentration when D-glucose was in excess.
• The method demonstrated reliable detection of dissolved oxygen.
• The developed methodology operates effectively within the 0-10 mg/L dissolved oxygen range.

Conclusions:

• The proposed method offers a sensitive and accurate approach for dissolved oxygen determination in flowing aqueous streams.
• This technique holds potential for real-time environmental monitoring applications.
• The combination of enzymatic reaction and chemiluminescence provides a robust analytical tool.