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Updated: Aug 9, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Continuous flow isoelectric focusing for purification of proteins
N Soulet1, H Roux-de Balmann, V Sanchez
1L.G.C., UMR 5503, Université Paul Sabatier, Toulouse, France. soulet@lgc.ups-tlse.fr
Abstract:
To estimate the feasibility of isoelectric focusing in a continuous mode, the conditions for obtaining stable and linear pH gradients were first investigated, using a mixture of carrier ampholytes. Then the operating parameters allowing the isoelectric focusing of bovine serum albumin as model protein were determined. A theoretical approach was used to find conditions minimizing the electrohydrodynamic effect, mainly responsible for protein dispersion, for the separation of bovine serum albumin and alpha-lactalbumin from bovine milk.
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