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Expression of CD45 isoforms lacking exons 7, 8 and 10
E Virts1, D Barritt, W C Raschke
1Sidney Kimmel Cancer Center, San Diego, CA 92121, USA.
Molecular Immunology
|August 7, 1998
Summary
This study reveals CD45 exon usage in T cells. The 789 isoform is dominant in cytotoxic and helper T cells, while other splice variants show varied expression patterns.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- CD45 is a crucial protein tyrosine phosphatase in T cell signaling.
- Alternative splicing of CD45 mRNA generates various isoforms with distinct functions.
Purpose of the Study:
- To investigate the CD45 exon usage patterns in different T cell subsets.
- To determine the relative abundance of CD45 splice variants in cytotoxic (Tc) and helper (Th) T cells.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) with exon-specific probes.
- Southern analysis.
- RNase protection assays.
Main Results:
- The 789 CD45 isoform is the major splice variant in most Tc and Th cell lines.
- The 89 isoform (lacking exons 4-7) is ubiquitously produced by Th cells but less abundant in Tc cells.
- In the BW5147 cell line, 789 isoform is ~75%, 89 isoform is ~24%, and isoform 9 (lacking exons 4-8) is ~1% of CD45 mRNA.
- Alternative splicing of exon 10 was also identified.
Conclusions:
- CD45 exon usage is differential across T cell types.
- The 789 isoform is a predominant splice variant in mature T cells.
- Alternative splicing of CD45 contributes to T cell functional diversity.