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A phage library-derived single-chain Fv fragment in complex with turkey egg-white lysozyme: characterization,
G Küttner1, T Keitel, E Giessmann
1Institut für Biochemie des Universitätsklinikums Charité der Humboldt-Universität zu Berlin, Abteilung Proteinstrukturforschung, Germany.
Abstract:
Using phage-display, an anti-turkey egg-white lysozyme single-chain Fv fragment was selected from a naive light chain variable region repertoire in combination with a heavy chain variable region 'mini library' of anti-hen egg-white lysozyme single-domain binders (Ward et al., 1989). Whereas the selected VH domain alone binds somewhat better hen egg-white lysozyme than turkey egg-white lysozyme, but both with comparatively low affinity, the specificity of VH is converted by addition of the VL domain. Thus, the single-chain Fv fragment is more specific for turkey egg-white lysozyme, with markedly increased affinities towards both lysozymes. The complex of single-chain Fv with turkey lysozyme has been crystallized and characterized by preliminary X-ray analysis.