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Related Experiment Videos

Proteolytic ratchets that control progression through mitosis

F M Townsley1, J V Ruderman2

  • 1Dept of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. fionat@warren.med.harvard.edu

Trends in Cell Biology
|August 8, 1998
PubMed
Summary

The anaphase-promoting complex/cyclosome (APC/C) controls cell division by degrading key proteins. A new family of WD40 repeat proteins may regulate APC/C activity during mitosis.

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Cell cycle-regulated proteolysis of mitotic target proteins.

Molecular biology of the cell·1999

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex or cyclosome (APC/C) is a crucial ubiquitin ligase complex regulating cell cycle progression.
  • APC/C activity is essential for the metaphase-anaphase transition and exit from mitosis.
  • It functions by ubiquitinating anaphase inhibitors and mitotic cyclins for degradation.

Purpose of the Study:

  • To review the known components and substrates of the mitotic ubiquitination machinery.
  • To discuss the role of the Fizzy/Cdc20p protein family in activating the APC/C.
  • To explore how APC/C activation by these proteins allows temporal control of substrate ubiquitination during mitosis.

Main Methods:

  • Literature review of existing research on APC/C and its regulators.

Related Experiment Videos

  • Analysis of protein structures and functions, particularly WD40 repeat proteins.
  • Discussion of experimental evidence supporting APC/C substrate ubiquitination mechanisms.

    • Main Results:

    • The APC/C complex is composed of multiple subunits and targets specific substrates for degradation.
    • The Fizzy/Cdc20p family of proteins acts as co-activators for the APC/C.
    • These co-activators are critical for the timely ubiquitination and destruction of APC/C substrates.

    Conclusions:

    • The APC/C is a central regulator of mitotic progression, controlling key transitions through substrate degradation.
    • The Fizzy/Cdc20p family plays a vital role in mediating APC/C activity.
    • Understanding these mechanisms provides insight into cell cycle control and potential therapeutic targets.