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Aspartate analysis in formulations using a new enzyme sensor

L Campanella¹, Z Aturki, M P Sammartino

¹Dipartimento di Chimica, Università di Roma La Sapienza, Italy.

Journal of Pharmaceutical and Biomedical Analysis

|April 1, 1995

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A novel biosensor directly analyzes aspartate in pharmaceuticals and aspartame in sweeteners. It utilizes an ammonia-sensitive electrode with immobilized L-aspartase enzyme for accurate detection.

Area of Science:

Biotechnology
Analytical Chemistry
Enzyme Technology

Background:

Accurate quantification of aspartate and aspartame is crucial in pharmaceutical and food industries.
Existing analytical methods may require complex sample preparation or lack direct detection capabilities.

Purpose of the Study:

To develop a direct biosensing method for aspartate and aspartame.
To create a robust and sensitive biosensor for routine analysis.

Main Methods:

Development of a biosensor incorporating an ammonia-sensitive gas-diffusion electrode.
Immobilization of L-aspartase enzyme onto a dialysis membrane using polyazetidine.

Main Results:

The developed biosensor enables direct analysis of aspartate and aspartame.

Related Experiment Videos

The immobilization technique ensures enzyme stability and sensor performance.

Conclusions:

The biosensor offers a promising tool for the direct and efficient analysis of aspartate and aspartame.
This technology can be applied to quality control in pharmaceutical formulations and sweetener products.