A simple and robust HPLC method for the determination of paroxetine in human plasma

The objective of this investigation was to establish and validate an HPLC method with UV detection for the determination of paroxetine in human plasma. The method was validated in the concentration range from 6 to 100 ng ml-1. The lower limit of reliable quantification (LLQ) was 6 ng ml-1. The extraction efficiency varied from 67.1 to 85.5% over this range. Accuracies calculated at three concentrations in each of three separate runs were between 99.4 and 109.6%, and precision data were between 1.86 and 9.1%. The means of the between-day precision at all concentrations were between 2.77 and 7.32%. The corresponding means of the accuracy data were in the region of 102.4 to 106.3%.