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Electrogenic bicarbonate secretion in mouse gallbladder

L C Martin¹, M E Hickman, C M Curtis

¹Department of Pharmacology, University of Cambridge, Cambridge, United Kingdom.

The American Journal of Physiology

|August 8, 1998

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Mouse gallbladder epithelium secretes bicarbonate via a conductive mechanism, stimulated by forskolin. This process, crucial for bile flow, involves bicarbonate ions and primarily an apical conductive pathway.

Area of Science:

Physiology
Epithelial Transport
Gastroenterology

Background:

Gallbladder epithelium plays a role in bile modification.
Understanding ion transport mechanisms is key to gallbladder function.

Purpose of the Study:

To investigate the mechanism of ion transport in mouse gallbladder epithelium.
To elucidate the role of bicarbonate and chloride ions in short-circuit current responses.

Main Methods:

Short-circuit current (Isc) technique applied to isolated mouse gallbladders.
Stimulation of adenylate cyclase system using forskolin, IBMX, and dibutyryl-cAMP.
Ion substitution experiments (bicarbonate, chloride) and pharmacological inhibition (acetazolamide, furosemide).

Main Results:

Related Experiment Videos

Forskolin and cAMP analogs significantly increased Isc, indicating active transport.
Bicarbonate ions were essential for forskolin-stimulated current, with removal reducing responses.
Apical chloride removal reduced current, but a significant portion remained, suggesting a conductive bicarbonate mechanism.

Conclusions:

Mouse gallbladder epithelium secretes bicarbonate via a predominantly conductive mechanism across the apical membrane.
Forskolin transforms the epithelium into an electrogenically secreting tissue.
The findings challenge explanations based solely on parallel chloride and bicarbonate exchange pathways.