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[Potato leafroll virus: purification, serologic detection and quantitative analysis in plants]

M Mehrad, H Lapierre, Y Maury

Comptes Rendus Hebdomadaires Des Seances De L'Academie Des Sciences. Serie D: Sciences Naturelles

|April 17, 1978

Summary

Potato Leafroll Virus (PLRV) was purified from potato plants. An Enzyme-Linked Immunosorbent Assay (ELISA) was developed to detect and quantify PLRV, aiding in disease management.

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Area of Science:

Plant Virology
Immunodiagnostics

Context:

Potato Leafroll Virus (PLRV) is a significant pathogen affecting potato crops worldwide.
Accurate detection and quantification of PLRV are crucial for effective disease management and yield protection.

Purpose:

To purify Potato Leafroll Virus (PLRV) from infected potato tissues.
To develop and validate an antiserum for PLRV detection.
To establish an Enzyme-Linked Immunosorbent Assay (ELISA) for quantifying PLRV concentration in plants.

Summary:

Potato Leafroll Virus (PLRV) was successfully purified from infected potato plants.
An antiserum against PLRV was generated, enabling the development of a direct Enzyme-Linked Immunosorbent Assay (ELISA).
The developed ELISA method provides a reliable means to detect PLRV presence and determine its concentration in potato plants.

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Impact:

Facilitates early and accurate diagnosis of PLRV infection in potato crops.
Enables precise assessment of viral load, informing disease management strategies.
Contributes to reducing crop losses associated with PLRV and improving potato cultivation practices.