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Integrin alpha v promoter activity in keratinocytes
1Department of Surgery, University of Texas Southwestern Medical Center, Dallas, USA.
The Journal of Surgical Research
|August 12, 1998
Summary
Investigating the alpha-v integrin gene promoter in keratinocytes revealed a key regulatory region. This region, homologous to the epidermal growth factor receptor promoter, binds specific proteins crucial for gene transcription.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Keratinocytes increase alpha-v integrin subunit expression during reepithelialization.
- The regulation of alpha-v integrin subunit expression requires further investigation.
Purpose of the Study:
- To investigate the promoter region of the alpha-v integrin subunit gene.
- To understand the regulatory mechanisms controlling alpha-v integrin expression in keratinocytes.
Main Methods:
- Cloning the human integrin alpha-v gene promoter into a luciferase reporter vector.
- Creating deletional mutants using PCR and performing computerized sequence analysis.
- Conducting gel-shift analysis with keratinocyte nuclear extracts and specific oligonucleotides.
Main Results:
- Deletion of a putative ets binding site (22 bp) reduced promoter activity by approximately 50%.
- A region with high homology to the epidermal growth factor receptor (EGFR) promoter was identified.
- Gel-shift analysis indicated binding of single-strand specific DNA binding proteins to motifs within this region, but not to the ets binding site.
Conclusions:
- Alpha-v integrin expression in keratinocytes is regulated by a promoter region homologous to the EGFR promoter.
- This regulatory region binds single-strand specific DNA binding proteins, suggesting their importance in controlling transcription.