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DNA fingerprinting of Mycobacterium tuberculosis from patients with and without AIDS in Rio de Janeiro
M E Ivens-de-Araujo1, F C Fandinho, A M Werneck-Barreto
1Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, RJ, Brasil.
Abstract:
Isolates of Mycobacterium tuberculosis derived from patients with AIDS from a single hospital in Rio de Janeiro were typed using a standardized RFLP technique detecting IS6110 polymorphism. Nineteen isolates were obtained from 15 different patients. Eleven distinct IS6110 patterns were found, with 4 banding patterns shared by 2 patients. The clustering value of 53% was much higher in comparison with clustering of M. tuberculosis strains from TB patients without clinical signs for HIV infection from randomly selected health centers. We present these results as preliminary data on M. tuberculosis strain polymorphism in Brazil and on the higher risk for recent transmission amongst patients with AIDS.