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Related Experiment Videos

Aqueous two-phase systems for protein separation. Studies on phase inversion

J C Merchuk1, B A Andrews, J A Asenjo

  • 1Department of Chemical Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel.

Journal of Chromatography. B, Biomedical Sciences and Applications
|August 12, 1998
PubMed
Summary

Phase equilibrium studies of the PEG 400-phosphate system reveal critical points and the impact of NaCl. Phase inversion and continuity depend on agitation, with two inversion points per tie line.

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Area of Science:

  • Physical Chemistry
  • Colloid Science

Background:

  • Phase equilibrium is crucial for understanding liquid-liquid systems.
  • Polyethylene glycol (PEG) and phosphate systems are relevant in various industrial applications.

Purpose of the Study:

  • To investigate phase equilibrium in the PEG 400-phosphate system.
  • To determine binodal lines, tie lines, and phase inversion points.
  • To analyze the influence of NaCl and fluid dynamics on phase behavior.

Main Methods:

  • Phase equilibrium studies including binodal and tie line determination.
  • Calculation of critical points on binodal curves.
  • Analysis of phase separation rates to determine phase continuity.
  • Investigation of agitation effects on phase inversion.

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Main Results:

  • Equilibrium binodal lines, tie lines, and phase inversion points were obtained for the PEG 400-phosphate system.
  • The presence of NaCl significantly shifted the binodal line in certain concentration ranges.
  • Phase continuity was found to depend on both mixture composition and fluid dynamics (agitation).
  • A range of ambiguity was identified with two phase inversion points per tie line, influenced by agitation intensity.

Conclusions:

  • Phase inversion and continuity in PEG 400-phosphate systems are complex, influenced by composition, NaCl concentration, and fluid dynamics.
  • The rate of phase separation is a reliable indicator of phase continuity.
  • The study provides a method to control experimental data consistency by analyzing the convergence of the ambiguity region towards the critical point.