Heat-shock proteins expression in fish central nervous system and its possible relation with water acidosis...

The expression of 70 and 60-kDa heat-shock proteins (HSP70 and HSP60) and glial fibrillary acidic protein (GFAP), determined by immunoblotting and immunohistochemical methods, was studied in fish neural tissue; moreover the possible correlation between the expression of these proteins in neural tissue and fish acidosis resistance was also examined. The HSP GFAP content was analyzed in four different teleostean fish species (gourami, carp, goldfish and trout) under control conditions and in carp under experimental conditions to induce HSPs expression. Under control conditions, HSP70 and HSP60 expression was similar in gourami, carp and goldfish, but gourami had the highest acidosis resistance; trout had the lowest HSP70 and 60 expression and lowest acidosis resistance. The HSP expression pattern was mainly neuronal under control conditions. HSP expression was induced in carp and the effect of this induction on acidosis resistance was studied. Two methods were used for HSP induction in carp: acid shock (2 h at 4.5 pH) and heat shock (2 h at 33 degrees C). A high acidosis resistance, although non-significant, was observed after heat pretreatment. An important HSP expression was detected in glial cells after induction. GFAP expression showed no association with acidosis resistance under either control or experimental conditions.