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Related Experiment Videos

tRNA nucleotide 47: an evolutionary enigma

N Cermakian1, W H McClain, R Cedergren

  • 1Département de biochimie, Université de Montréal, Québec, Canada.

RNA (New York, N.Y.)
|August 13, 1998
PubMed
Summary
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Transfer RNAs (tRNAs) lacking a nucleotide at position 47 but containing a U13:G22 base pair are preferred in databases. However, tRNAs with a C13:G22 pair and no nucleotide 47 show higher in vivo suppressor activity.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • A previous analysis suggested a correlation between the absence of nucleotide 47 (nt 47) in tRNA extra loops and the presence of a U13:G22 base pair in the D-stem.
  • This correlation implies potential functional differences in tRNA structures.

Purpose of the Study:

  • To evaluate the significance of the observed correlation between nt 47 and the D-stem base pair.
  • To determine the in vivo activity of tRNAs with varying nt 47 and D-stem base pair configurations.

Main Methods:

  • Assessing in vivo suppressor activity of engineered tRNA variants.
  • Conducting long-term growth experiments with competing Escherichia coli strains harboring different tRNA variants.

Main Results:

Related Experiment Videos

  • tRNAs lacking nt 47 but containing a C13:G22 pair exhibited higher in vivo suppressor activity than those with a U13:G22 pair and no nt 47.
  • Both C13:G22 and U13:G22 variants with no nt 47 supported growth of an Escherichia coli strain deleted for tRNA(Ala)GGC genes.
  • In long-term competition, the U13:G22 tRNA lacking nt 47 outcompeted the C13:G22 strain, irrespective of initial proportions.

Conclusions:

  • The preference for U13:G22 tRNAs lacking nt 47 in sequence databases is likely due to factors influencing extended growth or latency.
  • The in vivo suppressor activity does not fully explain the database prevalence of specific tRNA structures.
  • tRNA function and stability involve complex interactions beyond direct protein synthesis efficiency.