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Evidence for MAP kinase activation during mitotic division
1Groupe de Recherche Sur l'Interaction Gamétique (GRIG), CJF 9504 INSERM, Faculté de Médecine, Avenue de Valombrose, Cedex 02 France.
Journal of Cell Science
|August 14, 1998
Summary
Mitogen-activated protein (MAP) kinase activity, potentially from an ERK1-like protein, is present during sea urchin egg mitosis. Phosphatidylinositol 3-kinase (PI 3-kinase) may regulate MAP kinase deactivation during cell division.
Area of Science:
- Cell Biology
- Molecular Biology
- Developmental Biology
Background:
- Mitogen-activated protein (MAP) kinases regulate diverse cellular events.
- MAP kinase activation patterns differ between somatic cells and oocytes.
- Myelin basic protein (MBP) kinase activity during mitosis requires further investigation.
Purpose of the Study:
- Determine the origin and function of MBP kinase activity during mitosis in sea urchin embryos.
- Investigate the role of PI 3-kinase in MAP kinase regulation during early embryonic cell divisions.
Main Methods:
- Utilized anti-ERK1 antibodies and specific MAP kinase substrates.
- Performed in-gel phosphorylation experiments.
- Applied wortmannin, a PI 3-kinase inhibitor, to fertilized sea urchin eggs.
Main Results:
- Identified an ERK1-like protein contributing to MBP kinase activity during early mitotic divisions.
- Observed that PI 3-kinase inhibition prevents MAP kinase inactivation during egg division.
- Suggests a role for PI 3-kinase in the deactivation of MAP kinase.
Conclusions:
- An ERK1-like protein is involved in mitotic MBP kinase activity in sea urchin eggs.
- PI 3-kinase plays a role in the deactivation of MAP kinase during early embryonic divisions.
- MPF and MAP kinase activities may be interconnected for regulating early sea urchin embryo mitosis.