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DNA-Flow cytometric analysis of bladder TCC using paraffin-embedded tissues
L Wu1, A Lochhead, J Yang
1Oncology Research Centre, Institute of Oncology, Prince of Wales Hospital, University of NSW, Sydney, Australia.
Urologia Internationalis
|August 14, 1998
Summary
DNA flow cytometry (FCM) in bladder cancer reveals that diploid DNA patterns correlate with better prognosis. Aneuploid DNA patterns indicate malignancy and poorer outcomes, especially in recurrent tumors.
Area of Science:
- Oncology
- Pathology
- Urology
Background:
- Transitional cell carcinoma (TCC) is the most common type of bladder cancer.
- Accurate prognostic markers are crucial for managing TCC.
- DNA flow cytometry (FCM) analyzes DNA content in cells.
Purpose of the Study:
- To evaluate the prognostic value of DNA FCM in paraffin-embedded TCC tissues.
- To correlate DNA ploidy with histopathological grade, clinical stage, and patient outcomes.
Main Methods:
- Retrospective analysis of 239 TCC biopsy samples from 81 patients (1984-1994).
- DNA FCM performed on paraffin-embedded tissues.
- Comparison of DNA ploidy with tumor grade, stage, and clinical status (diagnosis, recurrence, mortality).
Main Results:
- Diploid DNA FCM results correlated with a better prognosis.
- Aneuploid DNA FCM results correlated with malignancy and poorer prognosis.
- Increasing tumor grade showed a trend towards higher DNA aneuploidy.
- Aneuploidy was significantly higher in malignant samples, recurrences, and fatal cases.
- Recurrent TCC samples exhibited significantly higher DNA aneuploidy than initial diagnosis samples.
Conclusions:
- DNA FCM is a valuable tool for assessing prognosis in TCC.
- Diploid DNA content suggests a favorable outcome, while aneuploidy indicates aggressive disease.
- DNA aneuploidy is associated with advanced tumor grade, recurrence, and mortality in TCC.