Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature induced structural changes of beta-crystallin and sphingomyelin binding

D Tang1, D Borchman

  • 1Department of Ophthalmology and Visual Science, University of Louisville, KY 40202, USA.

Experimental Eye Research
|August 14, 1998
PubMed
Summary

Alpha-crystallin’s interaction with sphingomyelin membranes changes lipid flexibility. Its thermal history dictates whether alpha-crystallin increases or decreases head group mobility in liposomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: The receptor for advanced glycation end products (RAGE) sustains autophagy and limits apoptosis, promoting pancreatic tumor cell survival.

Cell death and differentiation·2025
Same author

Correction: HMGB1 release and redox regulates autophagy and apoptosis in cancer cells.

Oncogene·2025
Same author

Epicardial adipose tissue associated with right cardiac chamber remodelling in patients with connective tissue disease evaluated by cardiac magnetic resonance: a cross-sectional study.

Clinical radiology·2025
Same author

[Clinical and pathological features and prognostic analysis of early-onset intrahepatic cholangiocarcinoma].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2025
Same author

[HER2 amplification and PD-L1 expression in invasive stratified mucin-producing carcinoma of the cervix: a clinicopathological analysis of eighteen cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2024
Same author

[Application of D-type stoma repair in parastomal hernia after permanent sigmoidostomy].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2024

Area of Science:

  • Biochemistry
  • Membrane Biophysics
  • Ocular Lens Physiology

Background:

  • Alpha-crystallin is crucial for ocular lens transparency and cataract formation.
  • Understanding alpha-crystallin's membrane interactions is key to its function and disease relevance.

Purpose of the Study:

  • To investigate the temperature-dependent binding of alpha-crystallin to sphingomyelin liposomes.
  • To analyze structural changes in alpha-crystallin and sphingomyelin induced by temperature.
  • To determine how alpha-crystallin binding affects lipid head group mobility.

Main Methods:

  • Utilized fluorescence probes NBD-PE and bis-ANS to monitor molecular interactions.
  • Studied temperature dependence of alpha-crystallin binding to sphingomyelin liposomes.

Related Experiment Videos

  • Analyzed structural changes in alpha-crystallin and sphingomyelin via fluorescence anisotropy.

    • Main Results:

    • Alpha-crystallin binding decreased NBD-PE anisotropy (increased head group mobility) at ≤37°C.
    • Preincubation near 60°C reversed this effect, decreasing NBD-PE anisotropy (reduced mobility) at 22°C or 37°C.
    • A structural transition in alpha-crystallin occurred around 47°C, influencing its effect on lipid flexibility.

    Conclusions:

    • Alpha-crystallin's impact on sphingomyelin liposome head group mobility is contingent on its structural state.
    • The thermal history of alpha-crystallin significantly modulates its interaction with cell membranes.
    • These findings offer insights into lens protein function and potential mechanisms in cataractogenesis.