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Phylogenetic placement of Trichonympha

J B Dacks¹, R J Redfield

¹Department of Zoology, University of British Columbia, Vancouver, Canada.

The Journal of Eukaryotic Microbiology

|August 15, 1998

Summary

Molecular data from hypermastigote protists, like Trichonympha, is now available. Phylogenetic analysis of the small-subunit ribosomal RNA gene supports their classification alongside trichomonads.

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Area of Science:

Microbiology
Molecular Biology
Protistology

Background:

Flagellated protists in the Class Hypermastigida have historically been classified solely based on morphology.
A lack of molecular sequence data has limited previous phylogenetic analyses of these organisms.

Purpose of the Study:

To obtain molecular sequence data for hypermastigote protists.
To establish a molecular phylogenetic framework for the Class Hypermastigida.
To compare molecular findings with existing morphological classifications.

Main Methods:

DNA was isolated from 350 cells of the hypermastigote Trichonympha, collected from Zootermopsis angusticollis hindgut.
Polymerase chain reaction (PCR) was used to amplify the small-subunit ribosomal RNA (SSU rRNA) gene.
DNA sequencing and phylogenetic analysis were performed on the amplified SSU rRNA gene fragments.

Main Results:

The DNA sequence of the amplified SSU rRNA gene from Trichonympha was obtained.
This sequence showed close relatedness to an unidentified gut symbiont from Reticulitermes flavipes.
Phylogenetic analysis positioned both sequences as a sister group to known trichomonads.

Conclusions:

Molecular data now supports the morphological classification of hypermastigote protists.
The study provides the first molecular phylogenetic insights into the Class Hypermastigida.
This research bridges a gap in understanding protist evolution using molecular techniques.

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