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Expression of phospholipase C-gamma 1 and its transcriptional regulators in breast cancer tissues
1Department of Surgery, Seoul National University, College of Medicine, Korea. dynoh@plaza.snu.ac.kr
Background:
PLC-gamma 1 is activated through direct interaction with growth factor receptor tyrosine kinase but little is known about the mechanisms controlling PLC-gamma 1 expression and its biological significance.
Materials And Methods:
Using immunoblotting, we evaluated PLC-gamma 1 protein overexpression in twenty breast cancer tissues. The expression of binding protein to GES1, GES2 and GES3, located in transcriptional regulator (GPE1) was found by electrophoretic mobility shift assay (EMSA). We also determined whether there was any correlation between prognostic factors (numbers of metastatic axillary nodes, histologic grade, c-erbB2, p53, and E-cadherin) and the overexpression of PLC-gamma 1 protein.
Result:
On immunoblotting, 17 of 20 breast cancer tissues showed overexpression of PLC-gamma 1, a result of which was corresponded to that of immunohistochemistry. The binding proteins to GES1, GES2 and GES3 were overexpressed only when PLC-gamma 1 protein overexpression was apparent. Positive expression of E-cadherin only was significantly associated with PLC-gamma 1 protein overexpression (x = 0.607, p = 0.045).
Conclusion:
GPE1 binding proteins might be the transcriptional regulator in PLC-gamma 1 overexpression and the relationship between expression of PLC-gamma 1 and E-cadherin would require further elucidation.
Insights
Phospholipase C-gamma 1 (PLC-gamma 1) protein is overexpressed in most breast cancers. Its expression correlates with transcriptional regulators and E-cadherin, suggesting a role in breast cancer progression.
Area of Science:
- Molecular Biology
- Oncology
- Biochemistry
Background:
- Phospholipase C-gamma 1 (PLC-gamma 1) is activated by growth factor receptor tyrosine kinases.
- Mechanisms controlling PLC-gamma 1 expression and its biological significance in cancer remain largely unknown.
Purpose of the Study:
- To investigate PLC-gamma 1 protein overexpression in breast cancer tissues.
- To identify potential transcriptional regulators of PLC-gamma 1.
- To explore correlations between PLC-gamma 1 expression and established prognostic factors.
Main Methods:
- Immunoblotting and immunohistochemistry to assess PLC-gamma 1 protein levels in 20 breast cancer tissues.
- Electrophoretic mobility shift assay (EMSA) to detect binding proteins to the transcriptional regulator GPE1 (GES1, GES2, GES3).
- Statistical analysis to correlate PLC-gamma 1 expression with prognostic factors like lymph node status, histologic grade, c-erbB2, p53, and E-cadherin.
Main Results:
- PLC-gamma 1 protein overexpression was observed in 17 out of 20 breast cancer tissues.
- Overexpression of GPE1 binding proteins (GES1, GES2, GES3) coincided with PLC-gamma 1 overexpression.
- Positive E-cadherin expression showed a significant association with PLC-gamma 1 protein overexpression (p=0.045).
Conclusions:
- GPE1 binding proteins may act as transcriptional regulators contributing to PLC-gamma 1 overexpression.
- The relationship between PLC-gamma 1 expression and E-cadherin warrants further investigation for its role in breast cancer.