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A second p53-related protein, p73L, with high homology to p73

M Senoo1, N Seki, M Ohira

  • 1Department of Molecular Oncology, Nippon Roche Research Center, Kanagawa, Japan. senomako@is.icc.u-tokai.ac.jp

Biochemical and Biophysical Research Communications
|August 15, 1998
PubMed

Insights

Researchers identified a new human gene, p73L, related to the p53 tumor suppressor. This gene, p73L, shows similarity to p73 and may have distinct roles in different tissues.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • The p53 protein is a critical tumor suppressor regulating cell division and death, frequently mutated in human cancers.
  • The p53-related gene, p73, was recently identified in a region linked to neuroblastoma pathogenesis.
  • A second human p53-related gene, p73L, exhibiting strong amino-acid similarity to p73, has been discovered.

Purpose of the Study:

  • To clone and characterize the novel human p53-related gene, p73L.
  • To determine the chromosomal location of the p73L gene.
  • To investigate the potential distinct roles of p73L and p73 in different tissues.

Main Methods:

  • Gene cloning and sequencing.
  • In situ hybridization for gene mapping.
  • Northern blot analysis for gene expression profiling.

Main Results:

  • The p73L gene was cloned and mapped to human chromosome 3q27-28.
  • p73L encodes a 586-amino acid protein with a DNA-binding domain highly similar to p53 and p73.
  • Distinct expression profiles of p73L and p73 mRNAs were observed in certain tissues.

Conclusions:

  • The identification of p73L expands the family of p53-related genes.
  • The distinct expression patterns suggest that p73 and p73L may possess separate and unique functions in various tissues.
  • Further research is warranted to elucidate the specific roles of p73L in cellular processes and disease.

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