Expression of CD44 isoforms in cultured human trabecular meshwork cells
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1Department of Ophthalmology, University of California, San Francisco, School of Medicine 94143-0730, USA.
Purpose:
To determine the expression of CD44 isoforms in cultured human trabecular meshwork (HTM) and to discuss their possible relationship with outflow facility.
Methods:
CD44 isoform expression in cultured HTM was qualitatively examined using immunohistochemistry and RT-PCR analysis.
Results:
Immunohistochemistry of cultured HTM showed intense staining with a CD44s antibody, and with antibodies against CD44 exon 7, exon 11-12 and exon 14. By RT-PCR, at least three isoforms of CD44 were expressed in HTM: CD44s, CD44v-III and CD44v-I.
Conclusions:
At least three isoforms of CD44 are expressed in the HTM. CD44 may play a role in binding and turnover of hyaluronic acid in the trabecular meshwork, thereby regulating outflow facility.
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