Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast elongation factor 3: structure and function

K Chakraburtty1, F J Triana-Alonso

  • 1Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226, USA.

Biological Chemistry
|August 15, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translational regulation by ABC systems.

Research in microbiology·2001
Same author

Evolutionary divergence of an elongation factor 3 from Cryptococcus neoformans.

Journal of bacteriology·2001
Same author

Functional discovery via a compendium of expression profiles.

Cell·2000
Same author

New chromatographic and biochemical strategies for quick preparative isolation of tRNA.

Nucleic acids research·2000
Same author

Three-dimensional cryo-electron microscopy localization of EF2 in the Saccharomyces cerevisiae 80S ribosome at 17.5 A resolution.

The EMBO journal·2000
Same author

Experimental prerequisites for determination of tRNA binding to ribosomes from Escherichia coli.

Methods in enzymology·2000

Elongation factor 3 (EF-3) is crucial for fungal protein synthesis, aiding ribosome function and cell growth. This essential protein facilitates tRNA release from the ribosome

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Elongation factor 3 (EF-3) is a unique protein essential for the fungal translational machinery.
  • EF-3 is a 116,000 molecular weight protein vital for yeast ribosome function in vitro and in vivo growth.
  • The YEF3 gene encoding EF-3 is essential for yeast survival.

Purpose of the Study:

  • To investigate the function and mechanism of Elongation factor 3 (EF-3) in yeast translation.
  • To elucidate the role of EF-3 in tRNA binding and ribosomal interactions.
  • To explore the potential existence of EF-3 homologs in higher eukaryotes.

Main Methods:

  • Analysis of deduced amino acid sequence of EF-3, identifying ATP-binding cassettes and lysine boxes.
  • In vitro translation assays using yeast ribosomes.

Related Experiment Videos

  • Chasing experiments to study tRNA dissociation rates from the ribosomal E-site.

    • Main Results:

    • EF-3 contains duplicated ATP-binding cassettes and lysine boxes crucial for ribosome interaction.
    • EF-3 stimulates aminoacyl-tRNA binding by facilitating deacylated tRNA release from the E-site.
    • EF-3 enhances tRNA dissociation from the E-site twofold, independent of tRNA affinity, requiring ATP hydrolysis.

    Conclusions:

    • EF-3 plays a critical role in the fungal translational process by modulating tRNA dynamics at the ribosome.
    • The mechanism of EF-3 involves ATP-dependent enhancement of tRNA dissociation from the E-site.
    • The presence and function of EF-3 homologs in higher eukaryotes remain an open question requiring further research.