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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA
B R Berridge1, J D Fuller, J de Azavedo
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA. bberridge@cvm.tamu.edu
Journal of Clinical Microbiology
|August 15, 1998
Abstract:
Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.
